"Our team is divided into 3 subgroups, each followed up on an analytical chemistry of another focus. The first of the subgroups solves the preparation and characterization of hyaluronan oligosaccharides and degradation products of hyaluronan derivatives. In this work, we solve analytical problems related to preparative low- or high-pressure chromatography, separation and identification of positional isomers present in the complex enzymatic hydrolyzate of the derivative, etc.  

The second subgroup in the company develops analytical chemistry as such. It introduces, verifies and validates analytical methods suitable for the identification and quantification of impurities in prepared products and, last but not least, performs selected routine determinations. In this work, the subgroup works closely with the control analytical laboratory and provides analytical support to all R&D colleagues and actively participates in solving problems related to the production of cosmetics, pharmaceuticals and final products of the company.

Colleagues from the last subgroup are actively involved in conducting and evaluating the pharmacokinetics of our products (ADME study - adsorption, distribution, metabolism, and excretion), preceded by the careful development of analytical methods focusing on the quantification of substances in biological matrices. In addition, we also focus on the molecular imaging of substances in cuts of selected tissues. Our entire group cooperates with a wide range of basic research departments.” 

Mgr. Martina Hermannová Ph.D., Head of Pharmacokinetics Team

Methods and technical background:

For the separation and quantification of impurities and degradation products, we use modern HPLC, UHPLC, and GC devices, always in combination with a diode array detector, an evaporative light scattering, refractometric or conductivity detector and/or mass spectrometer. To determine the molecular weight of hyaluronan, we use combinations of gel permeation chromatography with a light scattering detector (so-called GPC-MALLS). For chemical characterization of our derivatives and positional isomers of modified oligosaccharides of hyaluronan, we mainly use NMR and LC-MS. Quantification of analytes in biological matrices for pharmacokinetic studies is performed on LC-MS based on triple quadrupole and molecular imaging by desorption electrospray.

Key tasks and responsibilities:

• Design, implementation, and evaluation of pharmacokinetic studies
• Introduction of analytical and purification procedures for the quantification of substances in biological matrices
• Isolation and identification of impurities and degradation products based on derivatives of hyaluronan and its oligosaccharides; In particular with a view to distinguishing individual positional isomers
• Evaluation of mass and NMR spectra
• Activity in preparing patents, publications in impacted journals and presentations at national and international level
• Co-operation across research groups
• Education of younger scientists

What do we require?

• Tertiary education (Ph.D.) graduated in Analytical or Bioanalytical Chemistry or Pharmacy
• Experience in a similar position necessary
• Excellent orientation in scientific literature
• Knowledge of English (minimal level B2)
• Good knowledge of PC work with MS Office programs
• Ability to work in a team
• Responsibility, independence, thoroughness
• Interest in solving complex and multidisciplinary tasks

What do we offer?

• Support of personal development and education
• Flexible working hours with the ability to maximize harmonization of work and personal activities
• Foreign business trips
• One week of extra holidays after one year (overall 5 weeks)
• Background of a stable company with a tradition of more than 25 years in world markets
• Subsidized catering inside the company
• Financial contributions for children's recreation
• Discount on company products
• Interest-free housing loans
• Assistance in obtaining accommodation and loans for the purchase of own housing